Fig. 1.

Transactivation of BSEP by FXRα1 and FXRα2. Human, mouse, and rat bsep promoter reporters phBSEP(-2.6kb), pmBSEP(-2.6kb), and prBSEP(-2kb) were cotransfected into human hepatoma Huh 7 cells with either FXRα1 or FXRα2 expression plasmid, followed by treatment of the transfected cells with vehicle DMSO (0.1%) or CDCA (10 μM) for 30 h. Reporter transactivation levels as luciferase activities were detected with a dual-luciferase reporter assay system. The firefly luminescence was normalized based on the Renilla luminescence signal. The data are presented as mean ± SD of at least three separate experiments. *P < 0.05 between DMSO and CDCA-treated cells (Student t-test).