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. 2013 Nov;54(11):3030–3044. doi: 10.1194/jlr.M038323

Fig. 2.

Fig. 2.

The effects of relative expression levels of FXRα1 and FXRα2 on BSEP expression in human liver tissues and Huh 7 cells. (A) mRNA expression levels of BSEP and total FXR in healthy human liver tissues were determined by TaqMan real-time PCR and plotted against FXRα1/FXRα2 ratios using SigmaPlot version 11.0. The cycle time (Ct) values Ct24.92 for FXR and Ct27.12 for BSEP of one tissue sample were designated as a relative unit 1. (B) A series of mixtures of FXRα1 and FXRα2 plasmid DNA in various FXRα1/FXRα2 ratios (from 1.0, 1.2, 1.4, 1.6 to 1.8) with a constant amount of total FXR at 10, 25, or 50 ng were cotransfected with human BSEP promoter reporter in Huh 7 cells, followed by treatment of the transfected cells with CDCA for 30 h and subsequent detection of luciferase activity. FXRα1/FXRα2 ratios were plotted against total FXR and BSEP promoter transactivation as luciferase activities using SigmaPlot version 11.0. The left figure shows the level and position of each individual sample in the 3D diagram. The right figure is the smooth 3D model based on the data shown on the left.