Fig. 3.

Expression and localization of FXRα1 and total FXR in human liver tissues. Liver sections were subjected to immunohistofluorescent assays. FXRα1- and FXR-specific Abs and rabbit IgG as negative controls were used as primary Abs. Goat anti-rabbit Abs labeled with AlexaFluor 594 was used as the secondary Abs. Mounting medium containing DAPI (1.5 μg/ml) was used as nuclei counterstaining. Images were captured under a confocal microscope at a magnification of 40× with both fluorescent and DIC settings.