Fig. 5.

Isoform-dependent and species-specific transactivation of IR1a reporters by FXRα1 and FXRα2. (A) Human BSEP IR1a reporter pTK-3xhIR1a, (B) mouse/rat bsep IR1a reporter pTK-3xm/rIR1a, and (C) human BSEP ER2/IR1a reporter pGL3/p-2xhER2/IR1a were cotransfected with FXRα1 or FXRα2, followed by treatment of the cells with DMSO (0.1%) or CDCA (10 μM) for 30 h. Reporter luciferase activities were detected with the dual-luciferase reporter assay system. The data are presented as mean ± SD of at least three repeated experiments. *P < 0.05 between DMSO and CDCA-treated cells (Student t-test).