Fig. 2.

SID-LC-ECAPCI/SRM/MS quantification of uptake and distribution of 15-oxo-ETE and 11-oxo-ETE by (A) LoVo cells and (B) HUVECs over time. Cells were incubated at 37°C with 15-oxo-ETE (2 µM) or 11-oxo-ETE (2 µM) for 5, 30, 60, and 240 min. For each time point, cellular fractions were collected, extracted, spiked with [¹³C₂₀]15-oxo-ETE, and then derivatized with PFB. Each time point is plotted as the mean of triplicates with SEM.