Fig. 4.

SID-LC-ECAPCI/SRM/MS quantification of the ratio of cellular/media 11-oxo- and 15-oxo-ETE in (A) LoVo cells and (B) HUVECs. Cells were incubated with 15-oxo-ETE (10 µM), 15-oxo-ME (10 µM), 11-oxo-ETE (10 µM), 11-oxo-ME (10 µM), or 0.25% DMSO vehicle control for 60 min. Cellular and media fractions were collected, extracted, spiked with [¹³C₂₀]15-oxo-ETE, and then derivatized with PFB. Data are plotted as the means of triplicates with SEM of the ratio of analytes in the cellular over media fractions. Statistical significance was assessed by one-way ANOVA with post-hoc Bonferroni multiple comparison test (*P < 0.05).