Treatment of COS-7 cells transiently transfected with hLPLA2 gene with tunicamycin. COS-7 cells were transiently transfected with hLPLA2 gene for 48 h at 37°C. The transfectants were treated with or without 5 μM of tunicamycin for 24 h at 37°C. A: In LPLA2 assay, 10 μg protein of each cell homogenate was incubated for 1.5–5 min at 37°C with liposomes containing NAS as described in the Material and Methods. Error bars indicate SD (n = 3). C and T denote control (without tunicamycin) and tunicamycin, respectively. B: In Western blotting, 30 μg of protein of each homogenate was separated by SDS polyacrylamide gel electrophoresis, subjected to immunoblotting with an anti-hLPLA2 rabbit antibody, and visualized as described in the Material and Methods.