TABLE 1.

Affinity of cholesterol with TM peptide

Synthetic Peptide	Binding Buffer	Kd (nM)
TM-MHC-II	PBS with 5 mM CHAPS	31.25 ± 4.46
	PBS with 30% TFE	49.9 ± 7.72
	PBS with 2 mM PC	77.45 ± 6.45
Mutant TM-MHC-II	PBS with 5 mM CHAPS	469.1 ± 55.56
	PBS with 30% TFE	1,119 ± 113
	PBS with 2 mM PC	1002 ± 101