Fig. 4.

Demonstration of the ability of monoclonal antibody E06 to inhibit binding of OxLDL to J774 macrophages. J774 cells were added to 96-well microtiter plates as described in Fig 1A. Bt-CuOxLDL (1 μg/ml) was added to each well in the absence or presence of the indicated concentrations of competitors: LDL, CuOxLDL, BSA, and E06 (a monoclonal IgM natural antibody that binds to the PC phosphocholine moiety of oxidized phospholipid). Only E06 affects the ability of CuOxLDL to bind to macrophage scavenger receptors. The experiment shown is the composite of two experiments, which were normalized by the use of B/B₀ to describe the amount of ligand bound in the presence (B) or absence (B₀) of competitor. Each value is the average of triplicate determinations.