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. 2013 Nov;54(11):3215–3219. doi: 10.1194/jlr.D042226

Fig. 2.

Fig. 2.

HPLC chromatograms of sophorolipids produced with ARA supplementation. (A) Wild-type strain with 1.25 g/l ARA. (B) M30 strain with 1.25 g/l ARA. (C) M30 strain with total addition of 5 g/l ARA. Identification of peaks upon LC-MS analysis: 1) acidic mono-acetylated sophorolipids with a C20:4 fatty acid tail (MW 686); 2) acidic di-acetylated sophorolipids with a C20:4 fatty acid tail (MW 728); 3) lactonic di-acetylated sophorolipids with a C20:4 fatty acid tail (MW 710); 4) de novo formed lactonic di-acetylated sophorolipids with a C18:1 fatty acid tail (MW 688); and 5) de novo formed lactonic di-acetylated sophorolipids with a C18:0 fatty acid tail (MW 690).