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(A) HPLC purification of 19- and 20-HETE, detection at 210 nm, flow rate = 2 ml/min. (B) LC-MS/MS analysis of isolated 20-HETE by HPLC. (Top) Total ion chromatogram (TIC). (Bottom) Extracted-ion chromatogram (XIC) using 319.2/275.1. The TIC shows that there is very little impurity in the purified 20-HETE. XIC of 20-HETE was used to support the elution retention time and amount of 20-HETE.
