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. Author manuscript; available in PMC: 2013 Oct 9.
Published in final edited form as: Mol Ther. 2008 Mar 25;16(5):947–956. doi: 10.1038/mt.2008.50

Fig. 8. AAV vector-mediated knockdown of HD70 expression prevented degeneration of striatal neurons and motor behavioral impairment.

Fig. 8

Striatal sections from rats injected with AAV-shHD2 or -shEGFP followed by AAV-HD70, immunostained with antibodies to NeuN (a–d) or stained with Fluoro-Jade B (e,f). High power images were taken in the middle of the transduced areas of striatae. Stereological cell counts of NeuN-positive cells (g) or calbindin d28k-positive projection neurons (h) of the rat striatum transduced by AAV-shHD2 or -shEGFP followed by AAV-HD70. Mean±SEM (n=6) ipsilateral data are presented as a percentage of the number of cells counted in the contralateral hemisphere. NeuN-positive striatal cell survival: shHD2+HD70 = 91.7 ± 2.9%; shEGFP+HD70 = 60.1±4.2%. Calbindin-positive projection neuron survival: shHD2+HD70 = 98.1±6.8%; shEGFP+HD70 = 61.7±7.5%. Student’s t-test, ***p<0.001. (i) Spontaneous exploratory forepaw use in the cylinder test in rats that were injected with AAV-shHD2 or -shEGFP, followed by AAV-HD70. Mean±SEM (n=14) percentage of fore-paw use asymmetry, where negative scores indicate contralateral fore-paw impairment. For each group, results are presented for tests conducted prior to shRNA vector injection (Naïve), 13 days post-injection of shRNA vectors (shRNA) and 13 days post-injection of HD70 vector (shRNA+HD70). ANOVA: shEGFP+HD70 v other groups, all time points ** p < 0.01. Scale bars (a) 1mm, (b) 50µm, applies to all high magnification images.