FIG. 1.

Effect of Interferon (IFN)-α treatments on IPEC-J2 cells stimulated with H₂O₂ and tumor necrosis factor (TNF)-α. IPEC-J2 cells were treated with recombinant (r), or natural (n) IFN-α (2 preparations) at different concentrations (100, 25, 6, and 1 U/mL) or kept as untreated control. Then, they were submitted to oxidative stress by adding TNF-α and H₂O₂ (2 ng/mL and 1 mM, respectively); supernatants were harvested 18 h later. Results are expressed as a difference between release of IL-8 by IFN α-treated and control wells (A), and a concentration of IL-1β (B). *indicates a significant difference between control and IFN-α treatment (one-way ANOVA for repeated measures, P<0.05) **P<0.01.