FIG. 4.

IFN-α effects on cytokine gene expression in IPEC-J2 cells. IPEC-J2 cells were treated with LPS only at 1, 2, and 4 μg/mL or LPS (at the same concentrations)+rIFN-α at 1 and 100 U/mL. Untreated cells were used as a negative control. After 18 h of incubation, RNA from cells was extracted to evaluate cytokine gene expression. Data are expressed as 2^−ΔΔCt, where ΔΔCt=(ΔCt of untreated cells)- (ΔCt of treated cells). The asterisks indicate significant differences between control and IFN-α treatments (one way ANOVA). **P<0.01 ***P<0.001.