Fig. 2.

Selenoprotein expression in clones expressing IDI-C and IDI-T. (A) Levels and activity of selenoproteins in the IDI-T and IDI-C clones grown in normal serum containing medium. TR1 and GPx4 protein levels were measured by ELISA and normalised by total protein. GPx activity was measured and normalised by total protein content. (B) Real time PCR analysis of the IDI-T and IDI-C clones grown in normal serum containing media. Gene expression was measured for the selenoproteins TR1, GPx4, GPx1 and SelH and normalised by GAPDH (ratio). Values shown are means ± s.e.m.