Table 1.
Lineage Tracing Quantification
| Number of EYFP+ve Cells Counted | EYFP;∗SOX (Percent) | EYFP;Pit-1 (Percent) | EYFP;ACTH (Percent) | |||
|---|---|---|---|---|---|---|
| Embryonic lineage tracing | SOX2-CreERT2, induced at 11.5 and 12.5 dpc, harvested 18.5 dpc | 2426, n = 3 | 16, SD = 1.7 | 55.4, SD = 9.6 | 9.3, SD = 2.9 | |
| SOX2-CreERT2, induced at 13.5 dpc, harvested 18.5 dpc | 684, n = 4 | 37, SD = 10 | ||||
| EYFP;Pit-1-ACTH (Percent) | ||||||
| SOX9-CreERT2, induced at 11.5 and 12.5 dpc, harvested 18.5 dpc | 677, n = 7 | 37, SD = 9.6 | 41.4, SD = 3.8 | |||
| EYFP;LH (Percent) | EYFP;ACTH (Percent) | EYFP;PRL (Percent) | ||||
| Postnatal lineage tracing | SOX2-CreERT2, induced at P0/P2, harvested at 4 weeks | 4106, n = 4 | 35, SD = 3.2 | 39, SD = 5.6 | 3.2, SD = 0.7 | 0.9, SD = 1.2 |
| SOX2-CreERT2, induced at P0/P2, harvested at 8 weeks | 987, n = 2 | 42, SD = 2.5 | 6.7, SD = 0.2 | |||
| SOX9-CreERT2, induced at P0/P2, harvested at 4 weeks | 1897, n = 8 | 49, SD = 8.4 | 34, SD = 4.3 | 0.9 (n = 1) | 7, SD = 7.3 | |
| EYFP;SOX9;SOX2+ve (Percent) within EYFP;SOX9 | EYFP;SOX9;SOX2−ve (Percent) within EYFP;SOX9 | EYFP;Pit-1 (Percent) | ||||
| Adult lineage tracing | SOX9-CreERT2, induced at 8 weeks, harvested at 6 to 12 months | 436, n = 5 | 87, SD = 9.8 | 99, SD = 0.2 (n = 2) | 1.15, SD = 0.2 (n = 2) | 2 (n = 1) |
Double or triple immunofluorescence was performed for EYFP and progenitor or endocrine markers. EYFP+ve cells were counted in an average of 10 fields taken across the entire pituitary for each staining. Percentage of EYFP;marker+ve/EYFP+ve was calculated. Numbers are given when fewer than three pituitaries were used for a single staining. ∗SOX = SOX2 for Sox2CreERT2 samples and SOX9 for Sox9ires-CreERT2. See also Table S1.