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. 2013 Oct 15;382(2):496–503. doi: 10.1016/j.ydbio.2013.07.012

Fig. 1.

Fig. 1

Dvl cytoplasmic distribution in CE chordamesoderm cells is not polarised and is similar to that of dextran. (a) Stage 12.5 embryos were optically sectioned horizontally with respect to the anteroposterior axis, somewhat oblique to the equatorial plane of the gastrula embryo. The NSB runs as two near-parallel lines defining the notochord (Nt) in the middle from somatic mesoderm (S) on either side. All NSB directed ends (asterisks) were grouped versus the anti-NSB directed, anterior (A) and posterior (P). (E) is ectoderm, (M) is mesoderm. (b) A horizontal confocal image of a stage 12.5 embryo showing mosaic expression of myc-Dvl (green cells). beta-catenin staining reveals cellular outlines and the NSB. ((c)–(e)) Colocalisation of Dvl and rhodamine–dextran in a bipolar cell. (f) Bars represent the average fractional fluorescence measurements in the respective cell quadrants (excluding the nucleus) for Dvl and dextran in non-NSB-captured mediolateraly polarised cells at st.12.5. No statistically significant bias in the distribution of the Dvl/dextran ratio was found. Measurements are for 83 cells. Error bars are ±2×standard deviation.