Fig. 3.

Analysis of Dvl cortical localisation according to cellular location and distance from the NSB. Data from Fig. 2(a) and (b) are here shown broken down accoding to cellular location and distance (in cell diameters) from the notochord–somite boundary. ((a)–(h)) Fractional mean fluorescence of Dvl and dextran and their ratio in each cell domain. ((a)–(d)). Bipolarly protruding ((a) and (c)) and NSB-directed protruding cells ((b) and (d)) within 2 cell diameters from the NSB showing similarly significant enrichment in NSB-directed protrusions of both prospective notochord cells ((a) and (b)) and prospective somite cells ((c) and (d)). Bipolar cells that are 3 or more cell diameters away from the NSB (e) show only a mild enrichment at the NSB directed cell ends and NSB-directed cells (f) show no significant enrichment. Both bipolar and NSB-directed cells 5 or more cell diameters away from the NSB show no enrichment of Dvl in any domain. Error bars are ±2×standard deviation. (i) Schematic diagram summarising the statistically significant accumulations of Dvl/Dex fluorescence ratio in sub-categories of cells as indicated. The thickeness of the green line represents the accumulation of Dvl/Dex fluorescence. Black brackets are representing the pairwise comparisons between cellular domains (using multiple post hoc tests with a Bonferroni correction). Asterisks represent significance levels for each domain-pairwise comparison, where (^⁎⁎⁎) represents p<0.001, (^⁎⁎) is p<0.01 and (^⁎) is p<0.05. The distances of cells to the NSB are not to scale.