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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Cancer Ther. 2013 Jul 24;12(10):2135–2144. doi: 10.1158/1535-7163.MCT-12-1093

Figure 4.

Figure 4

Influence of PTEN depletion on DNA DSB induction and repair. (A) PTEN+/+ HCT116 (Neo 124) and PTEN−/− (#22 and #35) cells were plated 24 h in advance, after which they were subjected to γ-radiation (5 Gy). The level of DSBs was monitored by quantification of the average integrated fluorescence intensity per nucleus due to phosphorylation of histone H2AX as a function of time after 5-Gy irradiation. (B) Accumulation of DSBs by exposure of the HCT116 cells to 5 μM A12B4C3. Error bars represent ± SEM.