Skip to main content
. 2013 Oct 9;8(10):e76889. doi: 10.1371/journal.pone.0076889

Figure 1. Global gene expression profiles of human and mouse B lymphoma contain an abundance of deregulated genes.

Figure 1

(A) Representative tissue sections of human DLBCL (top) and mouse iMycBCL (bottom) stained with hematoxylin and eosin. The normal lymphoid tissue structure is effaced in both species by sheets of medium to large tumor cells that contain scant cytoplasm and round to polygonal nuclei with one to two nucleoli. Tingible body macrophages that harbor apoptotic bodies are abundant. Microscopic slides were read using an Olympus BX-51 light microscope equipped with UPLSAPO objectives (Olympus). The light temperature of the microscope bulb varied between 3000 and 3400 K. Imaging medium was air. Images were acquired with the help of a 40x objective (0.95 numerical aperture), DP2 digital camera (Olympus), and DP2-BSW imaging software (Olympus). Images were saved as TIF (tagged image file) data files and enhanced with respect to brightness, contrast and color balance using the Adobe Photoshop CS2 Version 9.0.2 software (Adobe Systems Inc). (B) Flow chart of global gene expression analysis of human and mouse lymphoma counterparts carried out in parallel and using the same statistical parameters (RMA, robust multi-averaging; ANOVA, analysis of variance; FDR, false discovery rate). Gene expression profiles of human DLBCL and normal B cells were compared on HG U133 microarrays. Gene expression profiles of mouse iMycBCL and normal B cells were compared on MG 430 microarrays.