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. 2013 Oct 9;8(10):e76890. doi: 10.1371/journal.pone.0076890

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© 2013 Han et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The two clades illustrate the likely enzymatic function of 14 L. cubeba unigenes. It also shows a distribution of the L. cubeba sequences throughout the two main clades of the tree. Vitvi Germacrene D (NP_001268213.1, (-)-germacrene D synthase, Vitis vinifera), Vitvi Caryophyllene (AEP17005.1, (E)-beta-caryophyllene synthase, Vitis vinifera), Maldo Germacrene D (AGB14625.1, germacrene-D synthase, Malus domestica), Frave Germacrene D (XP_004308410.1, (-)-germacrene D synthase-like, Fragaria vesca subsp. vesca), Artan Amorphadiene (AAF98444.1, amorpha-4,11-diene synthase, Artemisia annua), Eletr Copaene (ADK94034.1, alpha-copaene synthase, Eleutherococcus trifoliatus), Solly Germacrene C (XP_004242295.1, germacrene C synthase-like, Solanum lycopersicum), Actde Germacrene D (AAX16121.1, germacrene-D synthase, Actinidia deliciosa), Cicar Germacrene D (XP_004505471.1, (-)-germacrene D synthase-like, Cicer arietinum), Ricco Cadinene (XP_002523635.1, (+)-delta-cadinene synthase isozyme A, Ricinus communis), Vitvi Curcumene (ADR74200.1, beta-curcumene synthase, Vitis vinifera), Vitvi Cadinene (ADR74199.1, gamma-cadinene synthase, Vitis vinifera), Vitvi Valencene (NP_001268028.1, valencene synthase-like, Vitis vinifera), Vitvi Germacrene A (ADR66821.1, germacrene A synthase, Vitis vinifera), Citsi Valencene (AF441124_1, valencene synthase, Citrus sinensis), Solca Cascarilladiene (AAT72931.1, cascarilladiene synthase, Solidago canadensis), Goshi Cadinene (AAX44033.1, (+)-delta-cadinene synthase, Gossypium hirsutum), Theca Cadinene (EOY12645.1, delta-cadinene synthase isozyme A, Theobroma cacao), Warug Sesquiterpene (ACJ46047.1, sesquiterpene synthase, Warburgia ugandensis), Maggr Cubebene (B3TPQ6.1, beta-cubebene synthase, Magnolia grandiflora), Nicta Epi-Aristolochene (3M02.A, 5-Epi- Aristolochene Synthase, Nicotiana tabacum), Soltu Vetispiradiene (Q9XJ32.1, vetispiradiene synthase 1, Solanum tuberosum), Solly Vetispiradiene (AAG09949.1, AF171216_1, vetispiradiene synthase, Solanum lycopersicum), Litcu Ocimene (AEJ91554.1, trans-ocimene synthase, Litsea cubeba), Litcu Thujene (AEJ91555.1, alpha-thujene synthase, Litsea cubeba), Litcu Thujene/Sabinene (AEJ91556.1, alpha-thujene synthase/sabinene synthase, Litsea cubeba), Maggr Terpineol (ACC66282.1, alpha-terpineol synthase, Magnolia grandiflora), Maldo Ocimene (AGB14628.1, (E)-beta-ocimene synthase, Malus domestica), Phalu Ocimene (ABY65110.1, beta-ocimene synthase, Phaseolus lunatus), Citli Limonene (AAM53946.1|AF514289_1, (+)-limonene synthase 2, Citrus limon), Pontr Limonene (BAG74774.1, limonene synthase, Poncirus trifoliata), Cucsa Terpineol (XP_004161807.1, (-)-alpha-terpineol synthase-like, Cucumis sativus), Menaq Linalool (AAL99381.1, linalool synthase, Mentha aquatica), Cofar Limonene (CCM43927.1, limonene synthase, Coffea arabica), Vitvi Ocimene/Myrcene (ADR74206.1, (E)-beta-ocimene/myrcene synthase, Vitis vinifera), Vitvi Terpineol (NP_001268216.1, (-)-alpha-terpineol synthase, Vitis vinifera), Ricco Limonene (XP_002533355.1, (R)-limonene synthase, Ricinus communis), Queil Pinene (CAK55186.1, pinene synthase, Quercus ilex), Citun Ocimene (BAD91046.1, (E)-beta-ocimene synthase, Citrus unshiu), Citun Terpinene (BAD27259.1, gamma-terpinene synthase, Citrus unshiu), Artan Linalool (AAF13356.1|AF154124_1, (3R)-linalool synthase, Artemisia annua), Cinos Linalool (AFQ20811.1, S-(+)-linalool synthase, Cinnamomum osmophloeum), Vitvi Linalool/Nerolidol (ADR74212.1, (3S)-linalool/(E)-nerolidol synthase, Vitis vinifera).