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Cells were suspended (5 x 106 cells/mL) and placed in the upper well of 24-well transwell plates. The bottom wells contained either media alone, or media with recombinant CXCL12 (200 ng/mL) or CXCL13 (1000 ng/mL). Cells in the lower chamber were collected after 3 hours; percent migration is calculated relative to the input.
