Figure 5. Survival of liver MNC-derived cells in lethally irradiated mice.

(A) Hepatic MNCs (10⁶) from CD45.1⁺ mice were mixed with 10⁶ support BM cells from CD45.2⁺ mice and transferred into lethally irradiated CD45.2⁺ B6 mice. (B–C) Two months post-transfer, CD45.1⁺ cells in the recipient liver, spleen, and BM were gated, and the expression of CD3 and CD19 was analyzed (n = 3 mice/group). (D) Hepatic MNCs (10⁶) from CD45.1⁺ mice were transferred into CD45.1⁻ Rag-1^−/−Il2rg^−/− mice. (E) Two months post-transfer, CD45.1⁺ donor cells in the recipient liver, spleen, and BM were gated, and the expression of CD3e and CD19 were analyzed (n = 3 mice/group). All data are representative of 2 independent experiments.