Figure 3. Effect of increasing expression of tSSX on the localization of SS18-SSX.

Plasmid pEGFP-SS18-SSX2 (2 µg) was co-transfected in HEK293 cells with pDsRedmonomer-tSSX2 (0, 2, 4, and 6 µg) (A), or DsRedmonomer-tSS18 (0, 2, 4, and 6 µg) (B). The total DNA amount of transfection was complimented by pCMV-Tag2B empty plasmid without fluorescent protein (6, 4, 2, and 0 µg). The cells showing either a speckled pattern or a diffuse pattern of SS18-SSX2 localization were counted in 30 fields of fluorescence microscope, and the relative ratio of cells with speckled pattern was calculated (C). Scale bars indicate 10 µm.