Table 1. Bacterial strains and plasmids used in this study.
| Strains | Relevant characteristics a | Reference |
| E. coli Top 10 | F- mcrA Δ(mcrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 endA1 ara Δ139 Δ(ara,leu) 7697 galU galK λ − rpsL nupG λ − | Invitrogen |
| E. coli S17.1 | recA, hsdR RP4-2-Tc::Mu-Km::Tn7 | [22] |
| H. seropedicae SmR1 | Spontaneous Smr derived from type strain Z78 | [23] |
| H. seropedicae LPSEB | waaL::Km, Smr, Kmr | This work |
| H. seropedicae pLIG | SmR1 containing pLig, Smr, Tcr | This work |
| Azospirillum brasilense FP2 | N-nitrosoguanidine derived from type strain Sp7 | [24] |
| Plasmids and vectors | ||
| pTZHSwaaL | pTZ57 containing H. seropedicae SmR1 waaL gene, Apr | This work |
| pTZHSwaaLKM | pTZHSwaaL with waaL gene disrupted by Tn5 Km cassette, Apr, Kmr | This work |
| pSUPHSwaaLKM | waaL::Km Apr; Kmr; Cmr; | This work |
| pPWplig | pPW452 containing the promoter region of waaL upstream from lacZ, Tcr | This work |
| pUC4-KIXX | Apr; Kmr; cassete Tn5 Km | [25] |
| pSUP202 | Apr; Tcr; Cmr; mob site | [22] |
| pPW452 | Tcr, promoterless lacZ cassette | [26] |
| pTZ57R/T | Apr, TA cloning vector | Fermentas |
a
Ap = ampicillin; Km = kanamycin; Sm = streptomycin; Tc = tetracycline; Cm = chloramphenicol;