Table 2. Identification of isolated maize root lectin-like (MRL) proteins by MALDI-TOF spectrometry.
MRL-1 | MRL-2 | MRL-3 | |
PMF match | B4FN23_MAIZEPutative uncharacterized protein; Jasmonate-induced protein | B4F7S2_MAIZEPutative uncharacterized protein; Jasmonate-induced protein | B4G0K5_MAIZEPutative uncharacterized protein |
Sequence coverage | 28% | 18% | 41% |
Identity a | -gb|EES17612.1| hypothetical protein SORBIDRAFT_09g001880 [Sorghum bicolor] (63%)-gb|AAR20919.1| jasmonate-induced protein [Triticum aestivum] (41%)-gb|ABB51090.1| mannose-specific jacalin-related lectin [Oryza sativa Japonica Group] (40%) | -gb|EES17612.1|hypothetical protein SORBIDRAFT_09g001880 [Sorghum bicolor] (62%)-gb|AAR20919.1| jasmonate-induced protein [Triticum aestivum] (44%)-gb|ABB51090.1| mannose-specific jacalin-related lectin [Oryza sativa Japonica Group] (44%) | -gb|EER92327.1| hypothetical protein SORBIDRAFT_01g036580 [Sorghum bicolor] (92%)-gb|ABF95713.1| QXW lectin repeat family protein [Oryza sativa Japonica Group] (45%)-gb|ABW73994.1| lectin 2 [Euonymus europaeus] (42%) |
Protein alignment performed with NCBI PSI-Blastp (http://ncbi.nlm.gov/BLAST/).
Peptide mass fingerprint (PMF) comparisons to the database and sequence coverage data were obtained using the MASCOT program.