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. 2013 Oct 9;8(10):e77257. doi: 10.1371/journal.pone.0077257

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© 2013 Minde et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Intrinsic protein fluorescence spectra of APC-MCR measured in 1°C steps from 20°C to 70°C, indicated by a greyscale gradient from black to light grey. B, Comparison of intrinsic tyrosine fluorescence spectra normalised to the maxima. Experiment and colour code as in A. C Temperature dependence of intrinsic tyrosine fluorescence emission of MCR upon stepwise increase of temperature at 340 nm (black) and 304 nm (grey) normalised on maxima of emission.