Figure 2. siRNA mediated depletion of ER membrane components.

HeLa cells were treated with a control siRNA, or siRNAs against Sec62 (A), SRα (A) and Sec63 (B), subsequently harvested, and the relative levels of specific proteins determined by SDS-PAGE and immunoblotting. Levels of β-actin were used as a loading control and, following quantification, the relative amount of reach component was expressed as a percentage of the siRNA control sample. The values in the accompanying graphs show means (±s.e) for n ≥3, and in some diagrams the upper section of the error bars is omitted to preserve a uniform format.