Figure 6. Acute effects of BOF on food intake and circulating active ghrelin concentration.

(A) KKAy mice fed a standard diet were fasted for 24 hours and were administered BOF dissolved in distilled water via a stomach tube. After the single bolus BOF administration, 24-hour food intake was measured. (B) KKAy mice fed a standard diet were fasted 24 hours and were administered BOF dissolved in distilled water via a stomach tube. Two hours after the single bolus BOF administration, the mice were sacrificed in the fasted state, and blood samples were obtained. As a control group, we administered same amount of distilled water in the BOF group to mice under same conditions in each of the experiments. Values are presented as the means ± SEM, *P<0.05, **P<0.01 by Student’s t-test, vs. control group (n = 7–9).