Figure 4. Hexane fraction of American Ginseng (HAG) represses DLD-1 colon cancer cell migration in vitro.

Collagen type-I (15 µg/mL) coated transwell chamber were applied with 5×10⁴ DLD-1 cells (A/B) for 12 h. The lower chamber contains SFM/Serum (10%) or HAG (260 µg/mL in complete medium). (A) 5×10⁴ DLD-1 WT cells were applied to the upper chamber of the transwell membrane. (B) 5×10⁴ DLD-1 (transfected with mirvana miR-29b, 10 nM, 48 h) cells were applied to the upper chamber of transwell membrane. After 12 h incubation at 37°C, the cells migrated to the inside (lower membrane) of transwell membrane was counted using ImageJ software (7 random microscopic fields (100X) were evaluated for cell counting). (C) Depicts the representative picture of DLD-1 WT cell migration from each treatment. (D) Depicts the representative picture of DLD-1 (transfected with mirvana miR-29b, 10 nM, 48h) cell migration from each treatment. The background in the picture shows the 8 μm pore in the transwell membrane. *, indicates significant difference (pvalue <0.005) when compared to SFM.