Fig. 2.

Structure of the ParM filament. (A) and (B) Two views of the cryo-electron microscopy reconstruction of the ParM filament (EMDB ID: EMD-1980). (C) Relationship between the ice thickness and the filtered (red) or unfiltered (blue) intensity. The ice thickness is represented by $\text{ln}\left(\frac{I_{-\mathit{ef}}}{I_{+\mathit{ef}}}\right)$ in the ordinate. The distributed points of the filtered and unfiltered intensities over a range of ice thicknesses is fitted by linear regression, respectively. (D) Comparison of the fit of monomeric states of ParM in the EM reconstruction of the filament. ParM-AMPPNP (PDB ID: 4A61) and ParM-AMPPNP with the interacting region of ParR (PDB ID: 4A62) are fitted into a cropped segment of the EM reconstruction corresponding to a single ParM monomer. The table in (E) shows the statistics comparing the fits.