Figure 5.

Interdomain Interfaces in FUN-EGF3

(A) Packing interactions between the FUN (lilac) and EGF1 (green) domains, showing two views of opposite faces. Residues involved in interdomain packing are shown as spheres and are colored according to domain. Packing residues were identified using a 4 Å interatomic distance cutoff as described previously (Jensen et al., 2009).

(B) EGF2-EGF3 packing interactions. Residues from EGF2 and EGF3 involved in packing are colored light green and green, respectively.

(C) Sequence alignment of the FUN-EGF1 and EGF2-EGF3 regions. Cysteines and packing residues are colored yellow and red, respectively. The dotted line delimits the domain boundaries.

(D) Residues at interdomain interfaces that are substituted as a result of MFS-associated mutations (N57D and W71R in the FUN domain and P148S in EGF3). Further data for these mutations are given in Figure S2 and Table S2.