Table 1.

NMR Structure Calculation Statistics

	FUN-EGF3 (R45–E178)	FUN-EGF1 (A53–C111)	EGF2-EGF3 (C119–E178)
NOE-derived distance restraints
Total	2,599	1,559	1,040
Intraresidue	890	516	374
Interresidue	1,434	893	541
Sequential (| i-j | = 1)	571	340	231
Short-range (| i-j | < 5)	231	147	84
Long-range (| i-j | ≥ 5)	632	406	226
Ambiguous	275	150	125
Hydrogen bond restraints	30	18	12
Dihedral angle restraints
Total	137	69	68
φ	73	39	34
ψ	64	30	34
RDCs
1DNH	54	28	26
Total number of restraints	2,820	1,674	1,146
Restraint violations
Distance restraint violations > 0.5 Å	0	0	0
Dihedral angle violations > 5°	0	0	0
Rmsd from experimental restraints
Distance restraints (Å)	0.024 ± 0.001	0.025 ± 0.001	0.024 ± 0.003
Dihedral angle restraints (°)	0.338 ± 0.109	0.194 ± 0.141	0.408 ± 0.171
RDC restraints (Hz)	0.822 ± 0.074	0.755 ± 0.078	0.884 ± 0.110
Rmsd from idealized geometry
Bonds (Å)	0.004 ± 0.000	0.004 ± 0.000	0.003 ± 0.000
Angles (°)	0.513 ± 0.016	0.552 ± 0.016	0.501 ± 0.019
Impropers (°)	0.365 ± 0.007	0.417 ± 0.016	0.337 ± 0.015
Ramachandran plot
Residues in most favored regions (%)	80.5	81.8	81.9
Residues in additional allowed regions (%)	16.0	18.1	13.4
Residues in generously allowed regions (%)	1.6	0.1	1.7
Residues in disallowed regions (%)	1.9	0.0	3.0
Coordinate precision (rmsd; Å)
Backbone	N/A	0.586 ± 0.196	1.166 ± 0.288
Heavy atom	N/A	0.916 ± 0.246	1.820 ± 0.305

Statistics are given for full-length FUN-EGF3 and separately for the two domain pairs. Quality statistics were calculated using Xplor-NIH. Restraint violations and rmsd values are given as mean values per structure ± the SD. Ramachandran statistics were calculated using Procheck (Laskowski et al., 1996). N/A, not applicable.