FIGURE 3.

Inactive MARCH5 supports mitochondrial interconnectivity under neurodegenerative stress conditions. SH-SY5Y co-transfected with expression plasmids for PA-GFP and either MARCH5 or MARCH5^H43W were treated with 75 μM 6-hydroxydopamine for 6 h (A), 5 μM rotenone for 6 h (B), or 25 μMAβ peptide for 24 h (C) and mitochondrial interconnectivity was measured following activation of PA-GFP in a small area of the mitochondrial network. Shown is one representative picture and the average of three independent experiments with 15 cells each/condition. Statistical significance was analyzed using Student’s t-test with ** marking p < 0.01. Error bars represent SEM.