Effects of standard template mixtures on template detection by the non-edited probe. Ct values for the non-edited probe were measured for the non-edited template alone and for various combinations of A, B, and D templates, with or without the non-edited template. Ct Difference = Ct − Ct (non-edited template only). In the upper section, Ct Difference reflects cross-hybridization due to the presence of the mismatched templates: cross-hybridization = [1/(2Ct Difference)] × 100%. In the lower section, cross-hybridization is not calculated because binding of the probe to the matched template predominates. Each Ct value is the average of three replicates; results are expressed as mean ± SEM.