Table 3.

Effects of standard template mixtures on template detection by the non-edited probe. Ct values for the non-edited probe were measured for the non-edited template alone and for various combinations of A, B, and D templates, with or without the non-edited template. Ct Difference = Ct − Ct (non-edited template only). In the upper section, Ct Difference reflects cross-hybridization due to the presence of the mismatched templates: cross-hybridization = [1/(2^{Ct Difference})] × 100%. In the lower section, cross-hybridization is not calculated because binding of the probe to the matched template predominates. Each Ct value is the average of three replicates; results are expressed as mean ± SEM.

Template combination	Ct value	Ct Difference	Cross-hybridization (%)
No matching template
None	>35.000
A	>35.000	>10.000	<0.1
B	>35.000	>10.000	<0.1
D	29.383 ± 0.073	4.204 ± 0.094	5.084–5.789
A + B	>35.000	>10.000	<0.1
A + D	32.021 ± 0.692	6.842 ± 0.695	0.538–1.411
B + D	31.301 ± 0.250	6.112 ± 0.260	1.199–1.719
A + B + D	30.686 ± 0.560	5.507 ± 0.563	1.488–3.248
With matching template
Non-edited	25.176 ± 0.059	0.000 ± 0.084
Non-edited + A	25.298 ± 0.050	0.119 ± 0.078
Non-edited + B	25.521 ± 0.172	0.342 ± 0.182
Non-edited + D	25.083 ± 0.140	−0.095 ± 0.152
Non-edited + A + B	25.248 ± 0.331	0.069 ± 0.337
Non-edited + A + D	25.172 ± 0.127	−0.007 ± 0.140
Non-edited + B + D	25.283 ± 0.069	0.104 ± 0.091
Non-edited + A + B + D	25.401 ± 0.038	0.222 ± 0.070