Fig. 4. Down-regulation of specificity protein 1 (Sp1) by hesperidinon MSTO-211H. (A) The effect of hesperidin for 48 h on Sp1mRNA expression was determined by RT-PCR. The graphs indicated percentages of Sp1/β-actin. (B) The effect of hesperidin for 48 h on Sp1 protein expression was determined by western blotting. Actin was probed to determine the equal of the loading protein extract from each treatment. Data are shown as the mean ± SD of three independent experiments. The asterisks (*p<0.05) indicated a significant decrease in Sp1 protein in hesperidin-treated cells compared with untreated cells. (C) The time-dependent effect of hesperidin on Sp1 and apoptotic protein of PARP protein expression was performed in MSTO-211H cells were treated with 160 μM of hesperidin for 6 h, 9 h, 12 h, 24 h, and 48 h.
