Fig. 3. Effects of RSV on SIRT1 expression and activity. (A ,C) HT22 cells were incubated with or without 10 μM RSV for indicatedtime periods. (B, D) HT22 cells were incubated for 12 h with or without RSV at indicated concentrations. Western blot analysisfor SIRT1 expression (A, B) and SIRT1 activity assessment (C, D) were performed as described under Materials and Methods. Blots shown are representative of three independent experiments. Data are expressed as means ± SE from 3 and 4 experiments. *p<0.05 with respect to the untreated control group (Con).