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Enhanced uptake of DiO-labeled α-hEGFR-IL's in U87 mg and in U251 mg cell lines when compared to hIgG-IL's, or naked liposomes incubated with the cells for 2 hours. (A), (I) DiO-labeled liposomes (green) are only seen in cells incubated with anti-EGFR antibody conjugated liposomes. (C), (K) Verification of co-localization between green DiO-labeled α-hEGFR-IL's ((B), (J) red color) in merged overlays seen as yellow color. Cellular nuclei are visualized with DAPI. Scale bar = 50 μm.
