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. 2013 Sep 23;2013:964012. doi: 10.1155/2013/964012

Figure 2.

Figure 2

The effect of resolvin D1 on expression of COXs (COX-1 and COX-2) and PGE2 and PGD2 production at 6 hours in lung fibroblasts stimulated with LPS. ((a1), (a2), (c1), (c2)) HFL-1 cells were treated with resolvin D1 at 0, 10, 50, or 100 nM in the presence of LPS (1 μg/mL) for 6 hours. COX-2 protein was detected by western blot (∗ P < 0.05 versus control, # P < 0.05 versus LPS, LPS + resolvin D1 (10 nM) groups). No significant change in the protein expression of COX-1 was observed after treatment with LPS in HFL-1 cells. ((e1), (e2)) Supernatants from HFL-1 cells treated with resolvin D1 at 0, 10, 50, or 100 nM in the presence of LPS (1 μg/mL) for 6 hours were collected and PGE2 protein was measured by ELISA. Data are expressed as mean ± SE for each group (e1) (∗ P < 0.05 versus control, # P < 0.05 versus LPS group). PGD2 protein was also measured by ELISA. Data are expressed as mean ± SE for each group (e2) (∗ P < 0.05 versus control). ((b1), (b2), (d1), (d2), (f1), (f2)) We also dulicated our test in primary rat lung fibroblasts ((b1), (d1)) (∗ P < 0.05 versus control, # P < 0.05 versus LPS group). ((b2), (d2)) No significant change in the protein expression of COX-1 was observed after treatment with LPS in primary rat lung fibroblasts. (∗ P < 0.05 versus control, # P < 0.05 versus LPS group, (f1)). (∗ P < 0.05 versus control, (f2)). All experiments were repeated in triplicate.