Figure 4.

Splicing of SO miR-34b requires a purine-rich ESE. Schematic representation of the pcDNA3pY7 miR-34b mutants of the exon. The lines and black box correspond to the intronic miR-34b hairpin and exonic sequences, respectively. The mutated nucleotides of ESEmut are indicated. Lower panel shows the splicing pattern of pcDNA3pY7 miR-34b exonic mutants after transfection in HeLa cells. The identity of the bands is depicted on the right. Numbers below the panel indicate the percentage of splicing expressed as mean±SD of at least three independent experiments.