Figure 5.

Splicing of SO pri-miR-34b affects miRNA biosynthesis. Northern blot analysis of miR-34b and U6 snRNA. The analysis was performed on the AG dinucleotide mutants, on the 5′ss mutant described in Figure 3 (3′ss mut 1, 3′ss mut 2 and 5′ss mut) and on the mutant of the ESE shown in Figure 4 (ESE mut). Histograms show the fold increase of mature miR-34b normalized to U6. The abundance of miR-34b in the wild-type construct is set to 1.