Figure 3.

Pin1 is not required for GR trans-repression. A549 cells were treated with juglone for 1 h transfected with control or Pin1 siRNA and incubated for 48 h. Cells were then stimulated with TNFα (0.25 ng/ml) with 1 or 100 nM DEX. Cells were incubated for 16 h before the levels of NRE-luciferase (A and B) or TNFα-responsive genes (n = 3 for each) (C). IL-6 and (D). IL-8 were determined by qPCR. Graphs show mean (±SD) fold change in gene expression compared to controls (RQ). Statistical significance was determined using a GLM procedure determine the interaction between DEX and Pin1 (P > 0.05) on repression of TNFα-responsive genes (n = 3 for IL-6 and IL-8).