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. 2013 Jul 25;41(18):8526–8536. doi: 10.1093/nar/gkt644

Figure 1.

Figure 1.

CCAR1 interacts with AR and functions as an AR co-activator. (A) Endogenous interaction between CCAR1 and AR. LNCaP cells were treated with ethanol or 10 nM DHT for 16 h. Cell lysates were prepared and immunoprecipitated with normal IgG or anti-CCAR1. The immunoprecipitates were analyzed by immunoblot with the indicated antibodies. (B) CV-1 cells were transfected with pSG5.HA-AR (10 ng) and MMTV-LUC reporter (200 ng) in combination with various amounts (200, 400 and 800 ng) of pSG5.HA-CCAR1 and grown in medium containing or lacking 20 nM DHT before conducting luciferase assays on cell extracts. Data are means ± SD (n = 3). (C and D) Recruitment of CCAR1 to AR target genes. Cross-linked, sheared chromatin from LNCaP cells treated with or without 10 nM DHT (16 h) was immunoprecipitated with the indicated antibodies. qPCR analyses were performed using primers specific for the PSA enhancer and promoter (C) and TMPRSS2 enhancer (D). The results are shown as percentage of input and are means ± SD (n = 3).