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. 2013 Jul 25;41(18):8526–8536. doi: 10.1093/nar/gkt644

Figure 4.

Figure 4.

CCAR1 is required for optimal association of AR with target enhancers and AR-mediated chromatin looping and chromatin remodeling. (A, B and C) LNCaP cells expressing shNS or shCCAR1 were treated with or without 10 nM DHT for 16 h. Protein levels were monitored by immunoblot using the indicated antibodies (A). ChIP assays using the indicated antibodies were performed as described in Figure 1C. qPCR analyses were performed using primers specific for the PSA (B) and TMPRSS2 (C) enhancers. The results are shown as percentage of input and are means ± SD (n = 3). (D) 3C assays were performed using crosslinked, BstYI-digested chromatin from LNCaP cells expressing shNS or shCCAR1 treated with or without 10 nM DHT for 16 h. The ligated DNA was PCR amplified with primers as indicated. (E) FAIRE was conducted with LNCaP cells expressing shNS or shCCAR1 treated with or without 10 nM DHT for 16 h. qPCR analyses were performed using primers specific for the PSA and TMPRSS2 enhancers.