Figure 6.

The proposed role of CCAR1 in chromatin loading and assembly of AR transcription complex. CCAR1 is recruited to GATA2-dependent AR-binding sites at least in part through interaction with DNA-bound GATA2 and AR and stabilizes their association with DNA, thereby increasing their occupancy on AR-binding sites. The recruited CCAR1 possibly provides a physical link between DNA-bound AR and Mediator complex and facilitates recruitment of p160 co-activator complex, and thereby facilitating transcription complex assembly, chromatin looping and chromatin remodeling. For example, CCAR1 facilitates AR and GATA2 recruitment and stabilizes their occupancies on the distal PSA enhancer (ARE III) containing a GATA2-dependent ARE. CCAR1 also facilitates recruitment of Mediator complex and Pol II to AR-bound PSA enhancer (ARE III) and promoter (ARE I and ARE II). CCAR1 provides a physical link in AR transcription complex, thereby leading to chromatin looping and enhancing communication between the PSA enhancer and promoter.