Figure 2.

Altered function TA p73β mutants can activate transcription from non-canonical REs. Relative transactivation potentials of p73β alleles (WT, S139F and S260N) towards 12 non-canonical p53 REs comprising ¾- and ½-sites were determined using the yeast-based functional assay. The name and sequence of the 12 REs are shown. The core (GWWC) sequence is depicted in grey. Bars plot the mean fold of induction (normalized to OD₆₀₀) over the empty expression vector (pRS314) and standard deviations of four biological replicates, computed as in Figure 1.