Figure 4.

Twelve clinically relevant bacteria are resolvable by HRM. (a) Normalized and temperature calibrated database melt curves for each of the clinically relevant bacteria listed in Table 2 are resolvable, demonstrating the high sensitivity of HRM. (b) Difference curves of each bacteria using S. aureus as a reference. In previously work that did not include temperature calibrators or PCR optimal buffers, V6 amplicons of S. aureus, S. epidermidis and S. saprophyticus were not resolvable by HRM (14).