Table 1.

Calculated E-factors of the 10 EHDV2-IBA transcripts relative to the bovine transcriptome

The DiCoMPs approach is based on the calculated estimate of the signal-to-background ratio for the POI relative to the potential repertoire of proteins encoded in the cells being assayed. In this table, such calculation was performed with the 10 proteins encoded by EHDV2-IBA (top row) against the background of the bovine transcriptome. A cognate tRNA isoacceptor, expressed in bovine, was assigned to each codon of the viral or bovine mRNA sequences. Based on the dicodons present in these mRNA sequences, the estimated frequency of tRNA pairs was calculated under the simplifying assumptions of uniform transcript levels for all mRNAs and uniform translation levels. Presented are the enrichment factors (E-factor) for the different EHDV2-IBA proteins with the indicated tRNA pairs (first column), calculated according to the following formula: E-factor = (frequency of the given tRNA pair in the POI)/(estimated average frequency of this same tRNA pair in bovine transcriptome). In this table, the tRNA pairs with E-factors above 50 are highlighted in green.