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In-gel SHAPE data from HIV-1 RNA renatured using snap-cooling refolding conditions. (A) Ethidium bromide-stained gel slice showing the relative amounts of monomer and dimer present when RNA is renatured by slow-cooling as described in Materials and Methods (lanes 1 and 2), or using Lu et al. conditions (22) (lanes 3 and 4). (B) NMIA reactivities mapped onto the proposed structure, shown by colour according to the key. Data are an average of four independent experiments.
